DNA

Part:BBa_K1732007:Design

Designed by: Donna Lee   Group: iGEM15_Carnegie_Mellon   (2015-09-09)


pSB1C3-J23100-B0034-BFP-His-B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 285
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 607
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 518
    Illegal BsaI.rc site found at 683


Design Notes

Sequence is codon optimized for E.coli


Source

Sequence designed by Cheryl Telmer of Bruchez Lab in Carnegie Mellon University

References